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KMID : 0811720060100000339
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Korean Journal of Physiology & Pharmacology
2006 Volume.10 No. 0 p.339 ~ p.0
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THE EFFECT OF TUMOR NECROSIS FACTOR-ALPHA ON INTRACELLULAR CALCIUM IN MICE VENTRICULAR MYOCYTES
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Kupittayanant P
Eisner DA
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Abstract
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Tumor necrosis factor (TNF) is a proinflammatory cytokine that has a wide range of biological activities. It produced by a host in response to stress, infection, inflammation, tissue injury and shock, and has recently been recognized as a myocardial depressant substance. An increase in circulating TNF-alpha has also been implicated in a variety of cardiac
disease states. Several investigators have examined the effects of TNF-alpha on myocardial Ca handling; however, the mechanism underlying the effects of TNF-alpha on myocardial Ca handling remains elusive. The purpose of this work was therefore to examine: 1) the effect of TNF-alpha on ICa and contraction and 2) the effect of TNF-alpha on sarcoplasmic reticulum (SR) Ca content. Single ventricular myocytes were isolated from the hearts of adult male mice using a collagenase and protease digestion technique. The cells were loaded by fluo-3 for intracellular Ca measurements. Experiments were performed on the voltage-clamp using the perforated patch technique at room temperature. Superfusion solution was Tyrode solution. The data demonstrated that the effect of TNF-alpha is concentration-dependent and that the application of TNF-alpha induces a decrease in cardiac contraction. This occurs along with a decrease in the Ca transient and ICa amplitude. However, TNF-alpha has no effect on SR Ca content. Thus, the depressant effect of TNF-alpha is not beyond, but lies at the surface membrane of cardiac cells.
Source: Korean J Physiol Pharmacol.2006 Oct;10(Suppl II):245
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KEYWORD
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tumor necrosis factor, ventricular myocyte, calcium, sarcoplasmic reticulum
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